Lisure Achieves 5X Inline Protein Concentration Using Steady-State TFF (SSTFF)

Lisure Technology Has Just Unlocked a Milestone in Bioprocess Intensification (PI)—Real-Time 5X Inline Concentration of BSA Using Patented Steady-State Tangential Flow Filtration (SSTFF) Configuration on the M++ UltraPilot Platform

This success delivers what many in the industry have attempted but never realized: consistent, high-yield inline protein concentration under flow-through conditions.

Why This Matters

Inline concentration has long been recognized as a crucial enabler for next-generation biomanufacturing. However, attempts using Single-Pass TFF (SPTFF) by some industrial leaders have been hindered by gel layer formation, process instability, and poor yield at high concentration factors.

SSTFF changes the game. By maintaining a true steady state through tangential recirculation and advanced control, SSTFF eliminates the usual fouling risks while enabling robust, high-efficiency concentration inline and in real time.

The Result: 5X Real-Time Concentration with >95% Yield

In the latest study, a 0.2 mg/mL BSA solution was successfully concentrated to 1.0 mg/mL using SSTFF on a 0.1 m^2 cassette, achieving:

✅ >95% dynamic recovery based on off-line analysis
✅ Stable pressure/flow/plateaued UV absorbance (119–122 mAU) after starting-up
✅ Well controlled transmembrane pressure (1.0–1.26 bar)
✅ No membrane fouling over the test period
✅ Real-time steady-state inline operation

This kind of performance has not been previously demonstrated by any published single-path TFF approach.

Why Steady State > Single Path

It’s not the single pass that makes inline concentration viable—it’s the steady state.
SSTFF creates a controlled flow regime where concentration is stabilized across time, not just space. That’s what allows it to succeed.

Powered by M++ UltraPilot Platform

The experiment was executed on the M++ UltraPilot, Lisure’s fully integrated process intensification platform. With real-time UV detection, precision flow control, and modular configuration, the M++ UltraPilot provides the ideal environment for implementing novel processing strategies like SSTFF.

What This Means for the Industry

This breakthrough opens the door for seamless integration between chromatography and TFF and vice versa, furthermore than what SSTFF inline buffer exchange can do, reducing hold steps, minimizing tank usage, and accelerating process timelines. It marks a key advancement in the movement toward continuous and intensified biomanufacturing.

Join the Revolution

At Lisure, we believe process intensification is not a dream—it’s a system we can build together. SSTFF is just the beginning.

Follow us on LinkedIn or contact us to explore how SSTFF can be applied to your downstream platform.